The expression of CXCL13 in human serum, prostate tissue and cell lines. (A) Serum CXCL13 concentration in BPH patients and healthy controls. (B,C) The protein and mRNA expression of CXCL13 in BPH tissues and normal ones. (D–F) The mRNA and protein expression of CXCL13 in BPH-1 and WPMY-1 cells. Representative blots are shown. * p < 0.05, ** p < 0.01 and *** p < 0.001. (G,H) Immunofluorescence localization of CXCL13 in BPH tissues and normal ones. (I,J) Immunofluorescence localization of CXCL13 in BPH-1 and WPMY-1 cells. DAPI (blue) indicates nuclear staining and Cy3-immunofluorescence (red) indicates CXCL13 protein staining. Representative graphs are shown. All scale bars are 100 μm.