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. 2022 Dec 21;24(1):56. doi: 10.3390/ijms24010056

Figure 3.

Figure 3

Knockdown of CXCL13 inhibited proliferation, EMT and promoted apoptosis of BPH-1 cells via ERK1/2 and AKT pathway. (A–C) Knockdown efficiency of CXCL13 at mRNA and protein levels. (D,F) Statistical analysis of apoptotic rate (%) and percentages (%) of cells at each stage. (E,G) Flow cytometry analysis of cell apoptosis and cell cycle. (H) CCK8 assay of BPH-1 cells. *** means p < 0.001 between si-con and si-2, ### means p < 0.001 between si-2 and si-2+rHuCXCL13. (I,J) Western blot assay of cell-cycle-, cell-apoptosis- and pathway-related proteins. (K) Relative densitometric quantification of cell-cycle-, cell-apoptosis- and pathway-related proteins. (L) Western blot assay of EMT-related proteins. (M) Relative densitometric quantification of EMT-related proteins. * p < 0.05, ** p < 0.01, *** p < 0.001 and ns means no significant difference.