Table 5.
List of primary antibodies (All antibodies we used are rabbit antibodies).
| Antigens | Species | Dilution (IF) |
Dilution (IHC) |
Dilution (WB) |
Supplier |
|---|---|---|---|---|---|
| CDK2 | monoclonal | 1:1000 | CST, USA, Cat. 2546 | ||
| CDK4 | monoclonal | 1:1000 | CST, USA, Cat. 12790 | ||
| Cyclin D1 | monoclonal | 1:1000 | CST, USA, Cat. 2978 | ||
| BAX | monoclonal | 1:1000 | Abclonal, CHN, Cat. A19684 | ||
| BCL-2 | monoclonal | 1:1000 | Abclonal, CHN, Cat. A19693 | ||
| E-cadherin | polyclonal | 1:1000 | Abclonal, CHN, Cat. A3044 | ||
| N-cadherin | monoclonal | 1:1000 | Abclonal, CHN, Cat. A19083 | ||
| α-SMA | monoclonal | 1:100 | 1:1000 | Abclonal, CHN, Cat. A17910 | |
| Collagen Ⅰ | polyclonal | 1:100 | 1:1000 | Abclonal, CHN, Cat. A1352 | |
| TNF-α | polyclonal | 1:1000 | Abclonal, CHN, Cat. A0277 | ||
| IL-6 | polyclonal | 1:1000 | Abclonal, CHN, Cat. A0286 | ||
| IL-8 | polyclonal | 1:1000 | Abclonal, CHN, Cat. A2541 | ||
| CXCL13 | monoclonal | 1:500 | 1:500 | 1:1000 | Abcam, UK, Cat.ab112521 |
| pAKT | monoclonal | 1:1000 | Abclonal, CHN, Cat. AP0637 | ||
| AKT | monoclonal | 1:1000 | Abclonal, CHN, Cat. A17909 | ||
| pERK1/2 | monoclonal | 1:1000 | Abclonal, CHN, Cat. AP0472 | ||
| ERK1/2 | monoclonal | 1:1000 | Abclonal, CHN, Cat. A4782 | ||
| pSTAT3 | monoclonal | 1:1000 | Abclonal, CHN, Cat. AP0715 | ||
| STAT3 | polyclonal | 1:1000 | Abclonal, CHN, Cat. A1192 | ||
| GAPDH | monoclonal | 1:1000 | Abclonal, CHN, Cat. AC001 |