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. 2022 Dec 21;24(1):56. doi: 10.3390/ijms24010056

Table 6.

List of secondary antibodies used for Western blot.

Secondary Detection System Used Host Dilution Used Supplier
Anti-Rabbit-IgG (H + L)-HRP Goat 1:10000 (WB) Sungene Biotech, Tianjin, China, Cat. #LK2001
1:1000 (IHC)
Anti-Rabbit IgG (H + L), F(ab’)2 fragment (Alexa Fluor® (Irving, USA) 488 Conjugate) Goat 1:50 (IF) Cell Signaling Technology, Boston, USA, Cat. 4412
Hoechst 33342 (1 mg/mL) nucleic acid staining (DAPI) - 1:750 (IF) Molecular Probes/Invitrogen, Carlsbad, USA, Cat. A11007