Table 6.
Secondary Detection System Used | Host | Dilution Used | Supplier |
---|---|---|---|
Anti-Rabbit-IgG (H + L)-HRP | Goat | 1:10000 (WB) | Sungene Biotech, Tianjin, China, Cat. #LK2001 |
1:1000 (IHC) | |||
Anti-Rabbit IgG (H + L), F(ab’)2 fragment (Alexa Fluor® (Irving, USA) 488 Conjugate) | Goat | 1:50 (IF) | Cell Signaling Technology, Boston, USA, Cat. 4412 |
Hoechst 33342 (1 mg/mL) nucleic acid staining (DAPI) | - | 1:750 (IF) | Molecular Probes/Invitrogen, Carlsbad, USA, Cat. A11007 |