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. 2022 Dec 26;24(1):376. doi: 10.3390/ijms24010376

Figure 2.

Figure 2

Integrin αV is involved in the invasion of AGS cells cocultured with RAW264.7 cells. (A) The effect of macrophages on the adhesion of gastric cancer cells. After coculture of AGS cells and RAW264.7 cells for 24 h, cell adhesion to the extracellular matrix (ECM)-coated plate was analyzed using sulforhodamine B (SRB) dye (n = 3). (B,C) The effect of macrophages on the invasion of gastric cancer cells. After coculture of gastric cancer cells and RAW264.7 cells for 24 h, cell invasion was analyzed by SRB assay (n = 3). (D) Integrin αV expression induced by coculture of RAW264.7 cells with AGS or NUGC3 cells. Integrin αV expression was analyzed by Western blotting (three replicates). (E) The expression of integrin αV in AGS cells transfected with pCMV3−HA (empty vector) or pCMV3−Integrin αV−GFP (two replicates). (F,G) The effect of integrin αV on adhesion (F) and invasion (G) of AGS cells (n = 3). (H) The knockdown of integrin αV inhibited the increase in the invasion of gastric cancer cells induced by macrophages. After AGS cells were transfected with siScramble (SC) or siIntegrin αV for 24 h, the cells were incubated with RAW264.7 cells for 24 h. Cell invasion was analyzed using the SRB assay (n = 3). Student’s t−test was used for statistical analyses. The bars indicate the S.D.; the asterisks denote significant differences (*** p < 0.001, ** p < 0.01, * p < 0.05) between the means of two groups.