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. 2022 Dec 26;24(1):376. doi: 10.3390/ijms24010376

Figure 4.

Figure 4

VGLL1 is involved in TNF−α−induced expression of integrin αV. (A) The effect of VGLL1 on TNF−α−induced integrin αV expression. AGS cells were transfected with siScramble or siVGLL1 for 24 h and then the cells were treated with TNF−α for 24 h. Integrin αV expression was analyzed by Western blotting (four replicates). (B) The effect of VGLL1 S84 peptide on TNF−α−induced expression of integrin αV. After incubation with 20 µM control peptide or VGLL1 S84 peptide for 1 h, AGS cells were treated with TNF−α for 24 h. Integrin αV expression was analyzed by Western blotting (three replicates). (C,D) AGS cells were cocultured with RAW264.7 cells for 24 h in the presence of control or VGLL1 S84 peptide. (C) The effect of VGLL1 on integrin αV expression in AGS cells cocultured with RAW264.7 cells. Integrin αV expression was analyzed by Western blotting (three replicates). (D) The effect of VGLL1 on the invasion of AGS cells cocultured with RAW264.7 cells. Cell invasion was analyzed using the SRB assay (n = 3). ** p < 0.01, *** p < 0.001.