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. 2022 Dec 26;24(1):376. doi: 10.3390/ijms24010376

Figure 5.

Figure 5

TNF−α−induced integrin αV transcription is regulated through TEAD4. (A) AGS cells were treated with siScramble, siVGLL1, or siTEAD4, and then treated with TNF−α for 24 h. The mRNA expression of VGLL1, TEAD4, integrin αV, and RPL13A was analyzed by RT−PCR (three replicates). (B) The effect of VGLL1 and TEAD4 on integrin αV promoter activity. AGS cells were transfected with integrin αV−Luc and Renilla−Luc for 24 h and then cells were treated with siScramble, siVGLL1, or siTEAD4 for 24 h. After serum starvation for 24 h, the cells were treated with TNF−α for 24 h. The promoter activity of integrin αV was normalized by Renilla−Luc (n = 3). (C) In AGS cells treated with siScramble or siTEAD4, the TEAD4 binding site in the integrin αV promoter was analyzed by ChIP assay (two replicates). (D) The construction of various luciferase reporters under the control of the integrin αV promoter. (E) Integrin αV promoter activity containing modified TEAD4−binding sites was measured in AGS cells (n = 3). (F) Model of TNF−α−induced integrin αV expression. Student’s t−tests was used for statistical analyses. The bars indicate the S.D.; the asterisks denote significant differences (*** p < 0.001, ** p < 0.01, * p < 0.05) between the means of two groups.