Figure 2.

Complementation of the thermosensitive phenotype of CAB1-defective yeast strains by human PanKs. (A–C) Equal amounts of serial dilutions of cells (10⁴, 10³, 10², 10¹ cells/spot) from exponentially grown cultures of cab1Δ/cab1^G351S (A), cab1Δ/cab1^N290I (B), and cab1Δ/cab1^I291T (C) mutant strains carrying pFL38/CAB1 and pESC-URA3 vector containing the different hPANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. (D–F) Growth rates of cab1Δ/cab1^G351S (D), cab1Δ/cab1^N290I (E), and cab1Δ/cab1^I291T (F) strains carrying the empty vector (EV), CAB1 and human PANKs in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10⁴ cells/mL. Optical density measurements at 600 nm (OD₆₀₀) were determined at the indicated time points. Data for liquid assays represent three biological replicates (n = 3) and the plotted graphs represent the average ± SEM.