Figure 4.

Human PANK1–3 complement iron sensitivity of yeast strains with defective PanK activity. (A–C) The iron susceptibility of cab1Δ/cab1^G351S (A), cab1Δ/cab1^N290I (B), and cab1Δ/cab1^I291T (C) yeast strains carrying an empty vector or either yeast CAB1 or human PANK genes was determined using plate-based assays. Cells were spotted at the indicated cell densities onto SC medium without uracil and tryptophan with 2% galactose in the absence or presence of 7 mM FeSO₄. Cell growth was measured after 3 days of incubation at 28 °C and 37 °C. (D–F) Depit results of assays similar to those described in (A–C) but conducted in liquid culture with growth measured at 60 h post-inoculation. Liquid growth assays were conducted in triplicates (n = 3) and the plotted graphs represent the average ± SEM.