Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 26;24(1):366. doi: 10.3390/ijms24010366

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

UA activates PTEN to modulate the FAK/PI3K/Akt signaling pathway: (A) Breast cancer cell lines were treated with UA for 48 h. Western blot analysis with antibodies to tumor progression-relevant proteins as indicated. GAPDH was used to normalize the amount of protein in each lane. (B) Incubation of breast cancer cells with UA (30 μM) significantly decreases AGO2. (C) Increased PTEN negatively regulates the expression levels of (D) FAK and (E) PI3-kinase p110 (PI3K), and inactivates the phosphorylated (activated) forms of (F) PI3K (p-P85α^{Tyr467/Tyr199}), (G) Akt (p-Akt^Ser473), and (H) mTOR (p-mTOR²⁴⁸¹), and reduced level of (I) c-Myc in both cells treated with UA. Densitometric analyzes for Western blot were performed as described in Materials and Methods. Data are shown as mean ± SD for three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control group (Ctrl, 0 µM).

UA activates PTEN to modulate the FAK/PI3K/Akt signaling pathway: (A) Breast cancer cell lines were treated with UA for 48 h. Western blot analysis with antibodies to tumor progression-relevant proteins as indicated. GAPDH was used to normalize the amount of protein in each lane. (B) Incubation of breast cancer cells with UA (30 μM) significantly decreases AGO2. (C) Increased PTEN negatively regulates the expression levels of (D) FAK and (E) PI3-kinase p110 (PI3K), and inactivates the phosphorylated (activated) forms of (F) PI3K (p-P85α^{Tyr467/Tyr199}), (G) Akt (p-Akt^Ser473), and (H) mTOR (p-mTOR²⁴⁸¹), and reduced level of (I) c-Myc in both cells treated with UA. Densitometric analyzes for Western blot were performed as described in Materials and Methods. Data are shown as mean ± SD for three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control group (Ctrl, 0 µM).