Figure 4.

Composition of β7-expressing degranulating or intracellular cytokine-producing immune-reactive cells in uncultivated WB and after T-cell-enriched MLC using (A) leukemic and (B) healthy WB with or without Kit pretreatment as stimulator cells. Degranulation and intracellular cytokine production were quantified via flow cytometry in untreated and uncultivated WB as well as after 7 days of (T-cell-enriched) MLC with Kit-pretreated or untreated WB and IL-2. Results without LAA or SEB stimulation are given. Uncultivated cells in WB and cultivated cells after MLC from WB pretreated with Kit-M (MLC(M)) or without added Kits as control (MLC(CC)) are given. Mean frequencies ± standard deviation of immune-reactive cell subpopulations in (A) leukemic (AML/MDS) and (B) healthy samples; n—number of cases. Differences were considered as significant (*) when p ≤ 0.05. Double-sided arrows give (significant) differences between defined healthy and leukemic immune-reactive cell subtypes. Abbreviations of cell subpopulations are given in Table 1.