Figure 7.

Correlations of antileukemic functionality with frequencies of (leukemia-specific) β7-expressing immune-reactive cells before and after T-cell-enriched MLC using Kit-M-pretreated (vs. untreated as control) leukemic WB as stimulator cells. Cells were analyzed via flow cytometry before and after 7 days of (T-cell-enriched) MLC with Kit-pretreated or untreated WB and IL-2. Results of uncultured cells before MLC (MLC(UC)), and cells after MLC, from WB pretreated with Kit-M (MLC(M)) or without added Kits as control (MLC(CC)), are given. Lysis (improvement) is given as the best of value after MLC(M) (compared to MLC(CC)). (A) Clear positive correlation of frequencies of T_β7+/CD3⁺ with best blast lysis after MLC (with Kit-M-pretreated leukemic WB). (B) Significant positive correlation of frequencies of T_β7+/CD3⁺) with T_β7+IFNγ+/T_β7+ after MLC (with Kit-M-pretreated leukemic WB. (C) Significant positive correlation of frequencies of T_β7+107a+/T_β7 with improved blast lysis after MLC (with Kit-M-pretreated leukemic WB). r—correlation coefficient, p—significance, n—number of cases. Differences were considered as significant when p ≤ 0.05 and as highly significant when p ≤ 0.005. Abbreviations of cell subpopulations are given in Table 1.