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. 2022 Dec 27;24(1):447. doi: 10.3390/ijms24010447

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Confocal microscopy analysis of MSC treated for 0.5, 1, 3 and 6 h with CFSE⁺ EVs from participants with normal weight (Nw), overweight (Ow) and obesity. Pictures show the resultant recipient cells as proof of EV uptake. Nuclei were stained with Hoechst (blue). Scale bar: 100 μm. (B) Mean fluorescence intensity (MFI) quantification of CFSE ⁺ MSCs following the treatment with CFSE⁺ EVs. The table below shows the results from ANOVA 2-way analysis.