TABLE 1.
Infection of Chang conjunctiva epithelial cells with wild-type P. aeruginosa PAO-I results in a time-dependent breakdown of phosphatidylserine asymmetry in the cytoplasm membranea
| Period of expt (h) | % Annexin-positive cells
|
|
|---|---|---|
| No infection | Infection with PAO-I | |
| 0 | 8.9 ± 1.5 | 9.1 ± 2.7 |
| 3 | 12.3 ± 1.1 | 28.0 ± 4.8 |
| 6 | 14.6 ± 2.5 | 53.7 ± 4.9 |
| 9 | 24.0 ± 5.0 | 81.1 ± 4.3 |
a
Chang conjunctiva epithelial cells were infected with P. aeruginosa PAO-I for the indicated time, stained with FITC-annexin, and subjected to FACS analysis. Data are the means ± SD of results from three experiments.