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Purity of rat primary non-prefrontal cerebral cortical astrocytes. (A) Representative images of GFAP immunostaining in astrocytes. Red fluorescence indicates GFAP-positive astrocytes. Blue fluorescence indicates nuclei. (B) The ratio of GFAP-positive cells to nuclei represents the purity of astrocytes. The experiment was repeated three times, and more than 95% of cells were GFAP-positive. Scale bar: 75 µm. GFAP: Glial fibrillary acid protein.
