Figure 3.

Rg1 inhibited the opening of hemichannels in astrocytes induced by CORT. (A) Representative images describing EtBr uptake via hemichannels in each group. Red fluorescence indicates EtBr-positive astrocytes. Blue fluorescence indicates nuclei. (B) The ratio of EtBr-positive cells to nuclei represents the degree of EtBr uptake in astrocytes. CORT treatment increased astrocytic EtBr uptake, and all three concentrations of Rg1 (0.1, 1, and 10 μM) pretreatment reversed the effect. All data are shown as mean ± SEM (n = 3). Scar bar: 75 µm. *** p < 0.001. CORT, corticosterone; EtBr, Ethidium bromide.