Figure 4.
The effects of beta-amyloid isoforms on mitochondrial potential in bEnd.3 cells. Cells were incubated for 4 h (A,B), 24 h (C,D) and 48 h (E,F) with 10 μM of Aβ42, p-Aβ42 and iso-Aβ42. Flow cytometry was used to analyze changes in mean MitoProbe DilC fluorescence (A,C,E) and the number of cells with low mitochondrial potential (ψ) (B,D,F). Mean DilC fluorescence was normalized to control. The values in the control samples were taken as 100%. The mean ± SD in 3 independent experiments in triplicates is shown in the figure. The statistical difference between experimental groups was analyzed by one-way analysis of variance using the post hoc Tukey test for multiple comparisons. *—p < 0.05, **—p < 0.01 and ***—p < 0.001.