Figure 6.

The effects of beta-amyloid isoforms on the bioenergetic functions of bEnd.3 cells after 4 h incubation. Cells were incubated with 10 µM of Aβ₄₂, p-Aβ₄₂ and iso-Aβ₄₂. (A) Representative raw data of bioenergetic function. (B) Basal respiration, (C) ATP-linked respiration, (D) maximal respiration capacity, (E) spare capacity, (F) proton leak and (G) non-mitochondrial respiration are demonstrated. The results were calculated based on data obtained using bioenergetic functional analysis (for details, see Figure 5). The values in the control samples were taken as 1. All parameters were normalized to control. OCR is the oxygen consumption rate. FCCP is carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone. Each geometric figure (circle/square/up-pointing triangle/down-pointing triangle) in the histogram represents the result in an independent sample and corresponds to the Control, Aβ₄₂, p-Aβ₄₂ and iso-Aβ₄₂. The mean ± SD in 3 independent experiments in 4–8 replications is shown in the figure. The statistical difference between experimental groups was analyzed by one-way analysis of variance using the post hoc Tukey test for multiple comparisons. *—p < 0.05, **—p < 0.01 and ***—p < 0.001.