Figure 6.

Influence of the glycosylation status of ABCB1 protein on ABCB1 efflux activity of the fluorescent ABCB1 substrate Rh123 in DU145 and PC-3 DTXR cells. (a) DU145 and (b) PC-3 DTXR cells with or without pre-treatment with 100 ng/mL tunicamycin (TUN) for 96 h were incubated with 0.5 µg/mL Rh123 for 15 min. After several washing steps, RPMI 1640 medium was added and subsequently the cells were imaged in 10 min intervals. As examples, the first image (t₀) and images taken after 10 and 20 min are depicted for DTXR cells with or without tunicamycin pre-treatment (scale bar 50 µm). Longitudinal data were normalized to DTXR cells without tunicamycin pre-treatment at t₀ (black line in the graph). Data are depicted as mean ± SD of four to five independent biological replicates. Non-paired t-test with Welch’s correction was performed for comparison of DTXR cells with tunicamycin pre-treatment vs. DTXR cells without tunicamycin pre-treatment at each timepoint: * p < 0.05, ** p < 0.01, ns: not significant.