Figure 7.

Influence of tunicamycin-mediated deglycosylation of ABCB1 protein in combination with DTX on cellular viability of DU145 and PC-3 DTXR cells. DU145 and PC-3 DTXR cells with or without pre-treatment with 100 ng/mL tunicamycin (TUN) for 96 h were treated with a serial dilution of DTX. After treatment, the metabolic activity and the total adherent cell mass were determined by (a) the WST-1 assay and (b) the crystal violet assay, respectively, and IC₅₀ values for DTX were calculated. The adjacent graphs to the right depict IC₅₀ values calculated from individual experiments. All data were normalized to the indicated control (black line). Data are depicted as mean ± SD of four to six independent biological replicates. Non-paired t-test with Welch’s correction for comparison of two treatment groups: * p < 0.05, ns: not significant.