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Relative expression of PM H+-ATPase genes in cucumber roots exposed to Cd and/or AG. To determine the expression of PM H+-ATPase genes, real-time PCR analysis was performed as described in the Materials and Methods. The RNA was isolated from the control roots (control) and roots treated with 10 µM Cd (Cd) or 10 µM Cd and AG (Cd/AG). Values are the means of three replications. Error bars represent SD (asterisks indicate the significant differences, where ** p < 0.01).
