Table 3.
List of primers used in this study.
| Usage | Primer Name | Primer Sequence |
|---|---|---|
| Partial gene cloning |
CsPOR1 | F: CCAAGGCTCTAGCTGAAACA R: GAAAGGAGGAAGTGTCCAAGAT |
| Partial gene cloning |
CsTCS1 | F: CTGAATTGGTTTCACAGGGATTG R: TGTGGTCTTCGGTAGCTTTG |
| TRV virus testing |
pTRV1 | F: AAAGTGACTGGTGTGCCTAAA R: CTTGCAGAGCAGGAACTCTATC |
| TRV virus testing |
pTRV2 | F: CTACTACTACCAAGGCGAACAC R: GTCGTCAAGCCACTTCCTAA |
| Vector construction |
pTRV2-CsPOR1 | F: GAGGGTGGGTGATACCATATTC R: GCTGCTGTTTGTGCTCTTATAG |
| Vector construction |
pTRV2-CsTCS1 | F: GACACCTTCAATATACCCAGCTA R: ACTAGGATGATACTTGTGGTCTTC |
| qRT -PCR | qRT -PCR CsPOR1 | F: CCAAGGCTCTAGCTGAAACA R: GTCAAGTGAGGCAAGGTCTAA |
| qRT -PCR | qRT -PCR CsTCS1 | F: ACAAGCCCTCCTGTTGTAAG R: CCTCTTGGGATCTAGCATTGAG |
| qRT-PCR | qRT -PCR CsTIDH | F: GCGCCATCCGACTATGATTT R: CCACTACTTGTCCTCCATCTTTC |
| qRT-PCR | qRT -PCR CsSAM | F: CGATGAGACCGTGACAAATGA R: GAAGGGTTGAGGTGGAAGATG |
| qRT-PCR | Actin | F: CAGACCGTATGAGCAAGGAAAT R: GTGCTTAGGGATGCAAGGATAG |