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. 2022 Dec 21;24(1):125. doi: 10.3390/ijms24010125

Figure 4.

Figure 4

MyD88 siRNA attenuates Pg-LPS-induced migration of HASMCs. (A) The effect of MyD88 siRNA on cell number in Pg-LPS-stimulated HASMCs. MyD88 in HASMCs was knocked down (siRNA at 5 nM), stimulated with Pg-LPS (100 ng/mL) for 16 h, and evaluated using the MTT assay. n = 7 in each group. (B) Effect of MyD88 siRNA on DNA synthesis in Pg-LPS stimulation of HASMCs. MyD88 in HASMCs was knocked down (siRNA at 5 nM) and stimulated with Pg-LPS (100 ng/mL) for 16 h. Cells were labelled with BrdU for 16 h. n = 7 in each group. (C) Effect of MyD88 siRNA on the migration in Pg-LPS stimulation of HASMCs. Upper panels show the representative scratch wound healing assay. Lower panel shows the quantitative analyses of wound closure rate. MyD88 in HASMCs was knocked down (siRNA at 5 nM) and directly scratched. Cells were incubated with Pg-LPS (100 ng/mL) for 16 h. n = 5 in each group. Scale bar shows 100 μm. (D) Effect of MyD88 siRNA on the migratory property in Pg-LPS stimulation of HASMCs. Upper panels show the representative DAPI staining of migrated cells. MyD88 in HASMCs was knocked down (siRNA 5 nM). Cells were seeded in transwells and treated with Pg-LPS (100 ng/mL) for 16 h. n = 6 in each group. Scale bar shows 50 μm. * p < 0.05 vs. basal; ** p < 0.01 vs. basal. Data are presented as mean ± SE.