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. 2022 Dec 21;24(1):125. doi: 10.3390/ijms24010125

Figure 6.

Figure 6

TLR4 knockdown suppresses p38 MAPK, SAPK/JNK, and ERK activation in Pg-LPS induced HASMCs, while Myd88 knockdown suppresses p38 MAPK and SAPK/JNK activation in Pg-LPS-induced HASMCs but not of ERK. (A) Phosphorylation of p38 MAPK, SAPK/JNK, and ERK after Pg-LPS treatment. TLR4 in HASMCs was knocked down (siRNA at 5 nM), and cells were evaluated using Western blot analysis for p38 MAPK, SAPK/JNK, and ERK phosphorylation levels, 5 min after Pg-LPS treatment (100 ng/mL). Representative blots are shown. (B) Quantitative analysis of the p38 MAPK, SAPK/JNK, and ERK phosphorylation levels. TLR4 knockdown suppressed phosphorylation levels of Pg-LPS-induced p38 MAPK, SAPK/JNK, and ERK. n = 5 in each group. (C) Phosphorylation of p38 MAPK, SAPK/JNK, and ERK after Pg-LPS treatment. MyD88 in HASMCs was knocked down (siRNA at 5 nM), and cells were evaluated using Western blot analysis for p38 MAPK, SAPK/JNK, and ERK phosphorylation levels, 5 min after Pg-LPS treatment (100 ng/mL). Representative blots are shown. (D) Quantitative analysis of p38 MAPK, SAPK/JNK, and ERK phosphorylation levels. Myd88 knockdown suppressed phosphorylation levels of Pg-LPS-induced p38 MAPK and SAPK/JNK but not of ERK. n = 4 in each group. * p < 0.05 vs. basal; ** p < 0.01 vs. basal. Data are presented as mean ± SE.