Figure 1.

Influence of adrenoceptor activation on the number of lipid droplets (LDs)/positive cells in human MCF-7 breast cancer cells. Cells were treated with either (A) the non-selective adrenoceptor agonist adrenaline (1–100 µM) or (B) the non-selective β-adrenoceptor agonist isoprenaline (0.1–10 µM) for 24 h and 72 h. Results are expressed as percentage of control (solvent) and are presented as mean ± SD from 4−5 independent experiments. * p < 0.05, significantly different from solvent. (C) Representative microscopic images of LDs (orange fluorescence) in MCF-7 breast cancer cells without any treatment and after treatment, for 72 h, with either 100 µM adrenaline or 10 µM isoprenaline. Nuclei (blue fluorescence) were labelled with Hoechst 33342. Scale bar: 100 µm.