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. 2023 Jan 1;24(1):767. doi: 10.3390/ijms24010767

Figure 4.

Figure 4

Influence of cAMP-elevating agents forskolin and 8-Br-cAMP on the number of lipid droplets (LDs)/positive cells in MCF-7 breast cancer cells. (A) Results for the number of LDs/positive cells in MCF-7 cells after treatment with forskolin (0.1−10 µM) for 24 h or 72 h. (B) Results for the number of LDs/positive cells in MCF-7 cells after treatment with 8-Br-cAMP (1 and 10 µM) for 24 h or 72 h. Results are expressed as percentage of control (solvent) and are presented as mean ± SD from 5 independent experiments. * p < 0.05, significantly different from solvent. (C) Representative microscopic images of LDs (orange fluorescence) in MCF-7 breast cancer cells without any treatment and after treatment, for 72 h, with either 10 µM forskolin or 10 µM 8-Br-cAMP. Nuclei (blue fluorescence) were labelled with Hoechst 33342. Scale bar: 100 µm.