Skip to main content
. 2023 Jan 3;24(1):817. doi: 10.3390/ijms24010817

Figure 4.

Figure 4

LncRNA WDR11-AS1 binds to PABPC1, but does not alter PABPC1 expression, in chondrocytes. (A) Silver-stained gel of WDR11-AS1 interacting proteins isolated from SW1353 cells subjected to RNA pulldown assay using sense or antisense of WDR11-AS1. (B) Western blot analysis showed the binding of WDR11-AS1 to PABPC1 from RNA pulldown assay. (C) RT-qPCR from RIP analysis showed WDR11-AS1 in PABPC1 immunoprecipitates and IgG (control) from SW1353 lysates. (D) Prediction of subcellular localization of lncRNA WDR11-AS1 on the lncATLAS website. (E) Detection of subcellular localization of lncRNA WDR11-AS1 in primary chondrocytes by fractionation of nuclear and cytoplasmic RNA analysis. (F,G) Western blot analysis showed PABPC1 protein levels after WDR11-AS1 knockdown (F) or overexpression (G) in primary chondrocytes. * p < 0.05, PABPC1 vs. IgG (C); for (C,E–G), the measurement data are expressed as the mean ± standard deviation, and were analyzed by Mann–Whitney U test. The experiments were repeated three times.