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. 2023 Jan 3;24(1):874. doi: 10.3390/ijms24010874

Figure 3.

Figure 3

Expression of NFκB1 (A), NFκBIA (for IκBα, (B)), IκBKB (for IκK-β, (C)), PPPAR-α (D), PTGS2 (for COX-2, (E)) and IL-6 (F) genes in EA.hy926 cells preincubated for 48 h with 1 or 10 µM of fatty acid in a medium containing 0.1% of ethanol (Control) followed by incubation with a medium without fatty acids for 6 h. Cq values were normalized by the geometric mean of reference targets (YWHAZ and RPL13A genes). Bars are mean ± SD of 9 samples from 3 experiments. Data were analysed using the one-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, **** p < 0.0001; where asterisks are shown immediately above a bar they refer to the difference from the control and where asterisks are shown above a horizontal line they refer to the differences between the two groups indicated by that line. LA, linoleic acid; CLA9,11, cis-9, trans-11 linoleic acid; CLA10,12, trans-10, cis-12 linoleic acid.