Table 3.
Residue sidechain interactions between the nsp3 lumenal region and the nsp4 lumenal region (both without transmembrane regions). For each sidechain interaction, a tick (✓) in one of the three columns on the right indicates if it is also present in one of the models of the nsp3 and nsp4 lumenal regions complexed with and without transmembrane regions.
| Interaction Type | nsp3 Lumenal Region Residue | nsp4 Lumenal Region Residue | nsp3 with TMs + nsp4 with TMs | nsp3 with TMs + nsp4 without TMs | nsp3 without TMs + nsp4 with TMs |
|---|---|---|---|---|---|
| Hydrogen bond | Asn 2275 | Arg 2875 | ✓ | ✓ | ✓ |
| Asp 2296 | Lys 2830 | ✓ | ✓ | ||
| Asp 2296 | Phe 2881 | ✓ | ✓ | ✓ | |
| Ser 2303 | Cys 2851 | ✓ | ✓ | ||
| Ser 2303 | Gly 2814 | ✓ | ✓ | ✓ | |
| Ser 2303 | Gly 2815 | ✓ | ✓ | ✓ | |
| Gln 2308 | Ile 2961 | ✓ | ✓ | ✓ | |
| Gln 2308 | Arg 2985 | ✓ | ✓ | ||
| Ile 2309 | Cys 2989 | ✓ | ✓ | ||
| Ile 2309 | Gly 2987 | ✓ | ✓ | ✓ | |
| Ionic interaction | Asp 2296 | Lys 2830 | ✓ | ✓ | ✓ |
| Pi–pi interaction | Tyr 2270 | Phe 2881 | ✓ | ||
| Cation-pi interaction | Tyr 2270 | Arg 2875 | ✓ | ✓ | ✓ |
| Hydrophobic interaction | Leu 2298 | Ile 2873 | ✓ | ✓ | ✓ |