| Component | Concentration | Manufacturer | Catalog nr |
| Wnt3A | 50% v/v | In house cell line | / |
| R-spondin | 20% v/v | In house cell line | / |
| Noggin | 10% v/v | In house cell line | / |
| EGF | 50 ng/mL | Life Technologies | PMG8041 |
| A83-01 | 500 nM | Tocris | 2939/10 |
| SB202190 | 10 µM | Sigma-Aldrich | S7067 |
| Nicotinamide | 10 mM | Sigma-Aldrich | N0636 |
| n-Acetylcysteine | 1.25 mM | Sigma-Aldrich | A9165 |
| B27 | 1× | Life Technologies | 17504044 |
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