Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 3;24(1):881. doi: 10.3390/ijms24010881

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Colonogenic and differentiation potential of iMSCs in comparison with donor-matched MSCs. (A) Each individual iMSC line and their parental donor-matched MSCs were subjected to colony formation (CFU-F) assays and stained with crystal violet. Representative images for CFU-F assays (left panel) and their quantification (right) are shown (n = 3, mean ± SEM, *; p < 0.05). (B) Osteogenic differentiation of MSCs and iMSCs analyzed by Alizarin Red S staining for calcium deposition. Representative images for osteogenic differentiation (left) and quantification as determined by the absorbance at 405nm (right) are shown (n = 3, mean ± SEM, *; p < 0.05). (C) Adipogenic differentiation of MSCs and iMSCs analyzed by Oil- Red O staining for lipid droplets. Representative images for adipogenic differentiation (left) and the quantification by absorbance at 520nm (right) (n = 3, mean ± SEM, *; p < 0.05) are shown.