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. 2023 Jan 6;13:306. doi: 10.1038/s41598-022-27281-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Specificity and sensitivity of 8.9F antibody for rVSV-LASV detection. (a) 8.9F antibody specificity test. Average virus densities on 8.9F spots (n = 6 spots) obtained from SP-IRIS images of two different chips incubated with either rVSV-LASV or rVSV-EBOV, both at a concentration of 5 × 10⁴ PFU/mL. 8.9F antibody binds to rVSV-LASV whereas it does not show any significant binding for rVSV-EBOV chip. (b) Dilution series experiment for rVSV-LASV detection in FBS using 8.9F as the capture antibody (n = 6 spots). The lowest concentration detected is 10⁵ PFU/mL, however, actual LOD is closer to 10⁴ PFU/mL. Red dashed line represents the detection threshold obtained from 8.9F spots on a chip incubated with FBS alone and calculated as mean virus density plus three standard deviations. LOD experiment was performed at least three times in independent experiments.