Table 5. MS-spectra analyzed using PEAKS 7.5 in combination with MS2peptides-DB and http://proteome2pathogen.com/.
Species | Sample nr. | Number of unique peptide sequences after I to L replacement | Highest nr. of discriminative peptides on species level | Total nr. of peptides identified from the identified species | Identified microorganism |
Second best result on (first false positive)a | Second species identification (first false positive) |
---|---|---|---|---|---|---|---|
BM b | BM1343 | 6715 | 96 | 6559 | BM | 5 | B. gladioli |
BM | BM1345 | 6931 | 92 | 6730 | BM | 17 | BP |
BM | BM1347 | 5578 | 74 | 5405 | BM | 3 | B. oklahomensis |
BM | BM1349 | 5923 | 82 | 5764 | BM | 5 | B. gladioli |
BM | BM1352 | 6150 | 68 | 5958 | BM | 7 | BP |
BP c | BM1354 | 5355 | 259 | 5233 | BP | 6 | B. gladioli |
BP | BM1355 | 5266 | 331 | 5098 | BP | 2 | B. gladioli |
BP | BM1357 | 3774 | 173 | 3621 | BP | 7 | BM |
BP | BM1360 | 5792 | 268 | 5583 | BP | 5 | B. oklahomensis |
BP | BM1361 | 5795 | 257 | 5641 | BP | 7 | B. gladioli |
BP | BM649 | 6388 | 432 | 6223 | BP | 4 | B. cepacia |
a Second highest number of discriminative peptides on species level
b BM: B. mallei
c BP: B. pseudomallei.