Fig. 2. AR phosphorylation at serine 96 influences phosphorylation at other major phosphosites and is altered in SBMA cells.

(A to C) Western blot analysis of the phosphorylation status of AR12Q and AR55Q in HEK293T cells treated with vehicle (veh) or DHT (10 nM, 24 hours) (n = 3 to 4 biological replicates). (A) Left: HEK293T cells. Right: isogenic human iPSC-derived neural progenitor cells from an SBMA patient with 54Q and isogenic control with 23Q. Arrow indicates specific signal, and asterisk indicates nonspecific signal. Phosphorylated AR was detected using phospho-specific antibodies, and the total AR was detected with a specific antibody that recognizes AR independently of phosphorylation status. Graphs show means ± SEM, two-tailed Student’s t test. NS, nonsignificant.