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. 2023 Jan 6;9(1):eade1694. doi: 10.1126/sciadv.ade1694

Fig. 8. Haploinsufficiency or deletion of Cdk2 mitigates the phenotype of SBMA mice.

Fig. 8.

(A) Schematic of mouse cross and Western blot analysis of CDK2 levels in the brainstem of 8-week-old male wild-type (WT) and AR100Q mice (Cdk2 alleles are +/+, +/−, −/−) (n = 3 mice per genotype). (B) Western blot of phosphorylated AR in the brainstem of 8-week-old AR100Q/CDK2+/+, AR100Q/CDK2+/−, and AR100Q/CDK2−/− male mice (n = 2 to 3 mice per genotype). Graphs show means ± SEM. (C) Body weight analysis of mice with the indicated genotypes at 14 weeks [n = 14 (AR100Q/CDK2+/+), n = 10 (AR100Q/CDK2+/−), and n = 7 (AR100Q/CDK2−/−) mice per genotype]. (D) Changes in motor coordination (rotarod) and muscle strength (hanging wire) as mice age [n = 14 (AR100Q/CDK2+/+), n = 10 (AR100Q/CDK2+/−), and n = 7 (AR100Q/CDK2−/−) mice per genotype]. *P (AR100Q/CDK2+/+ versus AR100Q/CDK2−/−) < 0.05; $P (AR100Q/CDK2+/+ versus AR100Q/CDK2+/−) < 0.05; #P (AR100Q/CDK2+/+ versus AR100Q/CDK2−/−) = 0.08. (E) Muscle force measured using grip strength meter in 6-week-old AR100Q/CDK2+/+, AR100Q/CDK2+/−, and AR100Q/CDK2−/− mice (n = 3 mice per genotype). Graphs show means ± SEM, two-way (A) and one-way ANOVA followed by Tukey’s HSD test (B and E) or Fisher’s Least Significant Difference (LSD) (C and D).