TABLE 2.
Clonec | No. of fused aa | ORF (full length) | Protein identity | % Similarity | % Identity | SP or TM |
---|---|---|---|---|---|---|
2 | 74 | 2007 (169) | HI1085 | 89 | 79 | TM |
10 | 82 | 437 (227) | HI1603 | 89 | 80 | No |
15 | 378 | 1091 (404) | HI1701 | 66 | 49 | TM |
21 | 31 | 2011 (191) | HI1150 | 74 | 62 | SP |
42 | 145 | 543 (269) | HI0693 | 89 | 78 | SP |
51 | 65 | 802 (204) | HI0370 | 74 | 51 | TM |
53b | 43 | (367) | HI1236 | 24 | 44 | SP |
57b | 246 | (371) | HI1126.1 | 87 | 80 | TM |
66 | 25 | 1301 (136) | HI1628 | 58 | 46 | TM |
67 | 101 | 748 (185) | HI0389 | 65 | 50 | SP |
The table provides information on the number of amino acids in the protein fused to the PhoA, the ORF number (derived from the PEDANT database), the number of amino acids encoded by the full-length ORF, the identity of the protein, the identity to the same protein in the H. influenzae genome database, and whether the cloned protein has a signal peptide (SP) or transmembrane spanning (TM) segment.
DNA sequence not yet in the PEDANT annotated A. actinomycetemcomitans database so no ascribed ORF number is available.
Numbers in boldface type represent clones that were recognized by the rabbit antiserum to a saline wash of A. actinomycetemcomitans.