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Click reaction without the TBTA ligand. HPLC profiles of the [18F]FB-GR20 conjugate ((A)—before and (C)—after purification on NAP-5) and the [18F]FB-GOL1 conjugate ((B)—before and (D)—after purification on NAP-5). Conditions: Phenomenex Luna C8 column (5 µm, 4.6 × 150 mm); mobile phase: linear gradient starting with 90% eluent A (0.1 M triethylammonium acetate in water) and 10% solvent B (acetonitrile) with replacement by 70% eluent A and 30% eluent B at 35 min; flow rate 1 mL/min.
