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. 2022 Dec 30;28(1):294. doi: 10.3390/molecules28010294

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Click reaction with the TBTA ligand. HPLC profiles of precipitated GOL1 conjugate ((A)—immediately after conjugation and (C)—after 2 h) and precipitated GR20 conjugate ((B)—immediately after conjugation and (D)—after 2 h). Conditions: Phenomenex Luna C18 column (5 µm, 4.6 × 250 mm); mobile phase: linear gradient: starting with 100% eluent A (0.1 M triethylammonium acetate in water) and 2% solvent B (acetonitrile) with replacement by 65% eluent A and 45% eluent B at 20 min, flow rate 1 mL/min.