. 2001 Apr;69(4):2757–2761. doi: 10.1128/IAI.69.4.2757-2761.2001

TABLE 1.

Detection of OP-Tb activity in the bloodstream of infected rodents

Inhibitor^a	Mean activity^b (AFU · min⁻¹ · 25 μl of serum⁻¹) ± SD
Inhibitor^a	Uninfected	Infected
None	220 ± 28	2,247 ± 88
E-64 (10 μM)	188 ± 17	2,355 ± 109
Soybean trypsin inhibitor (100 μg · ml⁻¹)	169 ± 21	2,219 ± 199
Turkey ovomucoid (100 μg · ml⁻¹)	166 ± 12	2,303 ± 202
AEBSF (1 mM)	145 ± 12	166 ± 7
3,4-Dichloroisocoumarin (1 mM)	161 ± 11	154 ± 9

Inhibitor concentrations reflect the concentrations required for maximum inhibition of Cbz-Arg-Arg-AMC-hydrolytic activity in the sample. Increasing the inhibitor concentrations by up to double those reported here did not increase the degree of inhibition observed.

Trypanosome-free plasma from acutely infected rats and plasma from healthy rats was assayed for activity against Cbz-Arg-Arg-AMC (5 μM) in 50 mM Tris-HCl–1 mM DTT, pH 8, at 37°C.