TABLE 2.

Purification of OP-Tb from T. b. brucei-infected rat plasma

Step	Protein (mg)	Total activitya (pmol · s−1)	Sp act (pmol · s−1 ·  mg−1)	Purification (fold)	Yield (%)
Plasma	2,688	1,986	0.74	1	100
10–25% TPP fraction	1,506	1,109	0.74	1	56
Cibacron blue F3GA- Sepharose	442	1,071	2.42	3	54
Q-Sepharose	38	774	20.37	28	39
para-Aminobenzamidine– Sepharose	1.211	700	578.04	781	35
Phenyl-Sepharose	0.138	712	5,159.42	6,972	36
Poly-l-lysine–Sepharose	0.096	700	7,291.67	9,854	35
Sephacryl S-100 HR	0.056	677	12,089.29	16,336	34

^aActivity was determined against Cbz-Arg-Arg-AMC (5 μM) in 50 mM Tris-HCl–1 mM DTT, pH 8, at 37°C.