Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
Rims1tm3Sud/J (RIM1αβfl/fl) |
Kaeser et al., 2008 | RRID:IMSR_JAX:015832 | |
| Genetic reagent (M. musculus) |
Rims2tm1.1Sud/J (RIM2αβγfl/fl) |
Kaeser et al., 2011 | RRID:IMSR_JAX:015833 | |
| Genetic reagent (M. musculus) |
Erc1tm2.1Sud/J (ELKS1αfl/fl) |
Liu et al., 2014 | RRID:IMSR_JAX:015830 | |
| Genetic reagent (M. musculus) |
Erc2tm1.2Sud/J (ELKS2αfl/fl) |
Kaeser et al., 2009 | RRID:IMSR_JAX:015831 | |
| Genetic reagent (M. musculus) |
Unc13atm1.1Bros (Munc13-1fl/fl) |
Banerjee et al., 2022 | MGI:7276178 | |
| Genetic reagent (M. musculus) |
Unc13btm1Rmnd (Munc13-2-/-) |
Varoqueaux et al., 2002 | RRID:MGI:2449706 | |
| Genetic reagent (M. musculus) |
Unc13ctm1Bros (Munc13-3-/-) |
Augustin et al., 2001 | RRID:MGI:2449467 | |
| Cell line (Homo sapiens) | HEK293T cells | ATCC | Cat# CRL-3216; RRID:CVCL_0063 | |
| Recombinant DNA reagent | pFSW EGFP Cre | Liu et al., 2014 | pHN131014; lab plasmid code (LPC): p009 | |
| Recombinant DNA reagent | pFSW EGFP ΔCre | Liu et al., 2014 | pHN131015; LPC: p010 | |
| Antibody | Anti-RIM (rabbit polyclonal) | SySy | Cat# 140003; RRID:AB_887774; lab antibody code (LAC): A58 |
Immunofluorescence (IF) (1:500) |
| Antibody | Anti-PSD-95 (mouse monoclonal) | NeuroMab | Cat# 73-028; RRID:AB_10698024; LAC: A149 |
IF (1:500) |
| Antibody | Anti- Synaptophysin (guinea pig polyclonal) | SySy | Cat# 101004; RRID:AB_1210382; LAC: A106 |
IF (1:500) |
| Antibody | Anti-Munc13-1 (rabbit polyclonal) | SySy | Cat# 126103; RRID:AB_887733; LAC: A72 |
IF (1:500); Western blot (WB) (1:1000) |
| Antibody | Anti-Synapsin-1 (mouse monoclonal) | SySy | Cat# 106001; RRID:AB_2617071; LAC: A57 |
WB (1:4000) |
| Antibody | Anti-rabbit Alexa Fluor 488 (goat polyclonal) | Thermo Fisher | Cat# A-11034; RRID:AB_2576217; LAC: S5 | IF (1:200) |
| Antibody | Anti-mouse IgG2a Alexa Fluor 555 (goat polyclonal) | Thermo Fisher | Cat# A-21137; RRID:AB_2535776; LAC: S20 | IF (1:200) |
| Antibody | Anti-guinea pig Alexa Fluor 633 (goat polyclonal) | Thermo Fisher | Cat# A-21105; RRID:AB_2535757; LAC: S34 | IF (1:500) |
| Antibody | Anti-mouse peroxidase-conjugated (goat polyclonal) | MP Biologicals | Cat# 0855550; RRID:AB_2334540; LAC: S52 |
WB (1:10000) |
| Antibody | Anti-rabbit peroxidase-conjugated (goat polyclonal) | MP Biologicals | Cat# 0855676; RRID:AB_2334589; LAC: S53 |
WB (1:10000) |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622; https://www.mathworks.com/products/matlab.html | |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798; https://www.graphpad.com/scientific-software/prism | |
| Software, algorithm | Fiji/ImageJ | NIH | RRID:SCR_002285; https://imagej.net/software/fiji/downloads | |
| Software, algorithm | pClamp | Molecular Devices | RRID:SCR_011323; https://www.moleculardevices.com/products/software/pclamp.html | |
| Software, algorithm | MATLAB code for object recognition and analysis of 2D images | This paper | https://doi.org/10.5281/zenodo.6388196 | This code was adapted for 2D images from a previously generated code (Liu et al., 2018; Liu et al., 2022) and is freely accessible at zenodo.org |
| Software, algorithm | MATLAB code for hierarchical bootstrap | This paper | https://github.com/kaeserlab/Hierarchical_Bootstrap_Analysis_RB; Born, 2022 | This code was generated for this paper and is freely accessible at github.com |