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Western blot analysis of proteins associated with EFD induced apoptosis. Note: (A) The data demonstrate enhanced caspase 3 cleavage and downstream PARP, upregulation of pro-apoptotic P53 and BAX while downregulation of antiapoptotic NF-κB and BCL2. The loading control used was GAPDH. (B) Fold change comparative to control in cleaved PARP, Casp 3, P-53, BAX, BCL-2 and NF-κB expression after 48 h treatment with EFD.
