Figure 2.

Antiviral activity of berbamine hydrochloride against the ASFV in PAMs. (a) ASFV B646L and CP204L gene transcription levels were evaluated with an RT-qPCR; (b) ASFV production was evaluated with a TCID₅₀ assay; (c) ASFV p72 and p30 protein expression levels were evaluated with a Western blot; (d) ASFV p30 protein expression was evaluated with an IFA. Each datum represents the results of three independent experiments (means ± SD). Significant differences compared with the control group are denoted with *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 and ns denotes no significant difference.