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. 2022 Dec 25;28(1):170. doi: 10.3390/molecules28010170

Figure 6.

Figure 6

The effect of berbamine hydrochloride on the ASFV’s early stage. (a) The PAMs were pre-treated with berbamine hydrochloride for 4 h and then infected with the ASFV for 24 h; the ASFV B646L transcription level was evaluated with an RT-qPCR. (b) Attachment: the PAMs were infected with a mixture of the ASFV and berbamine hydrochloride at 4 °C for 1.5 h and incubated at 37 °C for another 24 h in the absence of drugs; internalization: PAMs were infected with the ASFV at 4 °C for 1.5 h and then treated with berbamine hydrochloride at 37 °C for 1 h, then the drug was discarded, and the solution was incubated at 37 °C for another 24 h in the absence of the drug. The ASFV B646L transcription level was evaluated with an RT-qPCR. Each datum represents the results of three independent experiments (means ± SD). Significant differences compared with the control group are denoted with *, p < 0.05; **, p < 0.01; ***, p < 0.001.