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. 2022 Dec 25;28(1):170. doi: 10.3390/molecules28010170

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of berbamine hydrochloride against the ASFV in PK-15 and 3D4/21 cells. The cell viability of berbamine hydrochloride in PK-15 (a) and 3D4/21(b) was evaluated by MTT assay and CC₅₀ was calculated by GraphPad Prism 8.0. Inhibition of berbamine hydrochloride against ASFV B646L and CP204L gene transcription in PK-15 (c) and 3D4/21(d) was evaluated by RT-qPCR. Each datum represents the results of three independent experiments (means ± SD). Significant differences compared with the control group are denoted with **, p < 0.01; ***, p < 0.001 and ns denotes no significant difference.