FIG. 2.

Antigen-specific responses by PBMC 1 week after the last immunization with the fusion proteins between Ag85B and ESAT-6. C57BL/6J mice were immunized three times with either Ag85B-ESAT-6 (A) or ESAT-6-Ag85B (B) emulsified in MPL-DDA. As a negative control, a group of mice received the adjuvant combination alone (C). The IFN-γ responses were measured in cell cultures pooled from eight animals in each group. Each point represents the mean of triplicate values ± standard error of the mean. The experiment was performed twice with similar results.