Extended Data Table 2 |.
MTX and antifolate resistance mutations in RFC
| Residue | Variants | Patient / Cell Line Derivation | Reference |
|---|---|---|---|
| S4 | P | Osteosarcoma patients | ref11 |
| A7 | V | Osteosarcoma patients | ref11 |
| E21 | K | Osteosarcoma patients | ref11 |
| R27 | H | Common coincident variant in osteosarcoma patients and cell lines | ref11 |
| V29 | L | Human cell line | ref41 |
| G44 | E | Murine cell line | ref35 |
| R | Human cell lines | ref36 | |
| E45 | K | Murine and human cell lines | ref38–41 |
| S46 | I | Human cell line | ref41 |
| N | Murine cell line, osteosarcoma patients | ref11,42 | |
| I48 | F | Murine cell line | ref37 |
| D56 | H | Acute lymphoblastic leukemia patients | ref84 |
| W107 (105 mouse) | G | Murine cell line | ref37 |
| A132 (130 mouse) | P | Murine cell line | ref34 |
| L143 | P | Human cell line | ref83 |
| A147 | V | Human cell line | ref83 |
| R148 | G | Human cell line | ref83 |
| F212 | del | Megaloblastic anemia patients | ref10 |
| S301 (297 mouse) | N | Murine cell line | ref85 |
| D522 | N | Acute lymphoblastic leukemia patients | ref84 |