| Antibodies |
|
| Rabbit anti-Cdk5 |
Rockland |
Cat# 200-301-163; RRID: AB_11182476 |
| Rabbit anti-SDHB |
Abcam |
Cat#154974; RRID: N/A |
| Rabbit anti-ChrA |
Abcam |
Cat#15160; RRID: AB_368477 |
| Rabbit anti-Tyrosine Hydroxylase |
Abcam |
Cat#6211; RRID: AB_2240393 |
| Rabbit anti-GFP |
Cell Signaling Technology |
Cat#2956; RRID: AB_1196615 |
| Rabbit anti-p35/25 |
Cell Signaling Technology |
Cat#2680; RRID: AB_1078214 |
| Rabbit anti-phospho-Thr172 AMPKα |
Cell Signaling Technology |
Cat#2535; RRID: AB_331250 |
| Rabbit anti-AMPKα |
Cell Signaling Technology |
Cat#2532; RRID: AB_330331 |
| Rabbit anti-phospho-Ser79 ACC |
Cell Signaling Technology |
Cat#11818; RRID: AB_2687505 |
| Rabbit anti-ACC |
Cell Signaling Technology |
Cat# #3662; RRID: N/A |
| Rabbit anti-phospho-Ser21/9 GSK3 |
Cell Signaling Technology |
Cat# 9331; RRID: AB_329830 |
| Rabbit anti-GSK3 |
Cell Signaling Technology |
Cat#9315; RRID: N/A |
| Rabbit anti-phospho-Ser15 p53 |
Cell Signaling Technology |
Cat#9284; RRID: AB_331464 |
| Mouse anti-p53 |
Thermo Fisher Scientific |
Cat# MA5-12557; RRID: AB_10989883 |
| Rabbit anti-p27Kip1 |
Cell Signaling Technology |
Cat#3686; RRID: AB_2077850 |
| Rabbit anti-phospho-Ser139 H2Ax |
Cell Signaling Technology |
Cat# 9718; RRID:AB_2118009 |
| Rabbit anti-p16INK4A |
Droteintech |
Cat#10883-1-AP; RRID: AB_2078303 |
| Mouse anti-Spectrin |
Millipore Sigma |
Cat#MAB1622; RRID: AB_94295 |
| Mouse anti-GAPDH |
Thermo Fisher Scientific |
Cat# 39-8600, RRID: AB_2533438 |
| Mouse anti-β actin |
Thermo Fisher Scientific |
Cat#PA5-78715; RRID: AB_2745831 |
| IRDye® 800CW Goat anti-Rabbit or anti-Mouse IgG |
LI-COR Biosciences |
Cat#926-32211; RRID: AB_621843, Cat# 926-32210; RRID: AB_621842 |
| IRDye® 680RD Goat anti-Rabbit or anti-Mouse IgG |
LI-COR Biosciences |
Cat# 926-68071; RRID:AB_10956166, Cat# 926-68070; RRID: AB_10956588 |
|
| Biological samples |
|
| Human adrenal tumor tissue microarray |
US Biomax Inc. |
Cat#AG801 |
| Human PC tissues |
Tumor bank (University of Alabama Birmingham); Dr.Karel Pacak [Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)] |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Roscovitine |
Dr. Laurent Meijer |
ManRos Therapeutics |
| MRT3-007 |
Dr. Laurent Meijer |
ManRos Therapeutics |
| MRT3-124 |
Dr. Laurent Meijer |
ManRos Therapeutics |
| CR8 |
Dr. Laurent Meijer |
ManRos Therapeutics |
| 25-16 |
Dr. Amarnath Natarajan (University of Nebraska) |
N/A |
| CVT-313 |
Selleck Chemicals |
Cat#S6537 |
| Metformin |
Sigma |
CAS#1115-70-4 |
| AICAR |
Sigma |
CAS#2627-69-2 |
| NF-56-EJ40 |
Med Chem Express |
Cat#HY-130246 |
| 2-DG |
Sigma Aldrich |
Cat# D6134 |
| Sulfo-MBS |
Sigma Aldrich |
Cat# 803227 |
| P-S65 PRKAG2 peptide - RKVDS*PFGC |
University of Texas Southwestern |
N/A |
| HBSS |
Gibco |
Cat#14175 |
| Gibco™ Fetal Bovine Serum |
Fisher Scientific |
Cat# 10-082-147 |
| Doxycycline |
Sigma Aldrich |
Cat# D9891 |
| Fluo-4AM |
Thermo Fischer Scientific |
Cat# F14201
|
| FuGENE® HD Transfection Reagent |
Promega |
Cat# E2311 |
|
| Critical commercial assays |
|
| 2-MET Plasma ELISA Fast Track |
Rocky Mountain Diagnostics |
Cat#BA E-8300 |
| Cell Counting Kit-8 |
Dojindo Molecular Technologies |
Cat#CK04-05 |
| Q5-site directed mutagenesis Kit |
New England Biolabs |
Cat# E0554S |
| DAKO Immunohistochemistry Kit |
Agilent Technologies, Inc. |
Cat# K064030-2 |
|
| Deposited data |
|
| Library of Cdk5 Phosphosites |
Carter et al. (2020)
|
https://www.phosphosite.org/Supplemental_Files.action
|
|
| Experimental models: Cell lines |
|
| hPheo1 (Wild-type, SDHB knockout) |
Dr. Hans Ghayee (University of Florida) |
Developed by Ghayee laboratory |
| MTT cells |
Courtesy of Dr. Karel Pacak (NICHD) |
Martiniova et al. (2009)
|
|
| Experimental models: Organisms/Strains |
|
| Mouse: PiggyBac PNMT-tTA |
Cyagen Biosciences |
N/A |
| Mouse: TetOp - P25GFP |
Meyer et al. (2008)
|
N/A |
| Mouse: PNMT-tTA x TetOp-P25GFP |
Dr. James Bibb (University of Alabama at Birmingham) |
N/A |
| Mouse: nu/nu |
Jackson laboratory |
JAX:002019 |
| Mouse: C.B-Igh-1b/IcrTac-Prkdcscid |
Taconic Biosciences |
TAC:cb17sc |
|
| Oligonucleotides |
|
| Primer: PNMT-tTA Forwardl: CAGTAGTAGATAAAGGGATGGGGAG |
This paper |
N/A |
| Primer: PNMT-tTA Reversel: GGGGCAGAAGTGGGTATGATG |
This paper |
N/A |
| Primer: PNMT-tTA Forward2: CAGGAGCATCAAGTAGCAAAAGAG |
This paper |
N/A |
| Primer: PNMT-tTA Reverse2: CACACCAGCCACCACCTTCT |
This paper |
N/A |
| gRNA sequence: SDHB: ATGGCAAATTTCTTGATACG |
Fisher Scientific |
N/A |
|
| Recombinant DNA |
|
| pLV[Exp]-EGFP:T2A:Puro |
VectorBuilder, Cyagen Biosciences |
Cat#VB160420-1011mqh |
| CMV pCalpain-sensor |
Addgene |
Cat#36182 |
|
| Software and algorithms |
|
| FIJI |
Schindelin et al., 2012
|
http://imagej.net/software/fiji/
|
| cBioportal |
Gao et al., 2013
|
http://www.cbioportal.org
|
| ShinyGO v0.61 |
Ge et al., 2020
|
http://ge-lab.org/go/
|
| GEPIA |
Tang et al., 2017
|
http://gepia.cancer-pku.cn/index.html
|
| UALCAN |
Chandrashekar et al., 2017
|
http://ualcan.path.uab.edu/
|
| XF96 Seahorse Wave |
Agilent Technologies |
https://www.agilent.com/zh-cn/product/cell-analysis/real-time-cellmetabolic-analysis/xf-software/seahorse-wave-desktop-software-740897
|
| ImageStudio |
LI-COR Biosciences |
https://www.licor.com/bio/image-studio/
|
| GraphPad Prism |
GraphPad Software, Inc |
http://www.graphpad.com/scientific-software/prism/
|
|
| Other |
|
| CODA noninvasive BP system |
Courtesy of Dr. David Pollock, University of Alabama at Birmingham |
N/A |
| Production of P-S56 PRKAG2 antiserum |
SouthernBiotech, Birmingham, AL |
N/A |
| Magnetic Resonance Imaging |
Institutional Research Core, University of Alabama at Birmingham |
N/A |
| Agilent Seahorse XFe96 Analyzers |
Bio-Analytical Redox Biology (BARB) Core, UAB Department of Nutrition Sciences |
https://www.uab.edu/shp/drc/cores/barb-core
|
| Bioluminescence imaging (IVIS Lumina III) |
Small Animal Imaging Shared Facility, Department of Radiology & Comprehensive Cancer Center, University of Alabama at Birmingham |
N/A |
