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. 2023 Jan 7;26(2):105944. doi: 10.1016/j.isci.2023.105944

Figure 1.

Figure 1

Susceptibility of Caco-2 cells to SARS-CoV-2 infection

(A) Percentage of SARS-CoV-2-infected cells detected in Caco-2 cell lines from different sources infected with different SARS-CoV-2 isolates at a MOI 0.01 as determined by immunostaining for the viral spike (S protein) 48 h postinfection.

(B) Cytopathogenic effect (CPE) formation in SARS-CoV-2 (MOI 0.01)-infected Caco-2 cell lines from different sources as determined 48 h postinfection.

(C) Susceptibility of Caco-2-F03 cells to a broad range of SARS-CoV-2 isolates after different times of cultivation. Cells had been frozen at passage 14 and were resuscitated and cultivated for a further 30 passages. SARS-CoV-2 susceptibility was determined by immunostaining for S 48h after SARS-CoV-2 (MOI 0.01) infection 3 and 30 passages post-resuscitation.

(D) ACE2 and TMPRSS2 levels in Caco-2-F03, Caco-2A, and single-cell derived clones from Caco-2A.

(E) Susceptibility of Caco-2A clones to selected SARS-CoV-2 isolates as indicated by immunostaining for S and CPE formation in SARS-CoV-2 (MOI 0.01)-infected cells 48 h postinfection.

(F) Correlation of S staining and CPE formation with cellular ACE2 levels. Correlations were determined using the Pearson correlation coefficient.

Results are expressed as the mean ± standard deviation.