REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS-CoV-2 spike protein antibody	Sino Biological	Cat: 40592-R190
peroxidase-conjugated anti-rabbit secondary antibody	Dianova	SKU: 111-035-045
Alexa Fluor 488 anti-rabbit secondary antibody	Invitrogen	Cat # A32731; RRID: AB_2633280
GAPDH	Trevigen	#2275-PC-100; RRID: AB_2107456
SARS-CoV-2 NP	Sino Biological	#40143-R019; RRID: AB_2827973
ACE2	Abcam	#ab15348; RRID: AB_301861
TMPRSS2	Abcam	#ab92323; RRID: AB_10585592
IRDye®800CW Goat anti-Rabbit	LI-COR Biotechnology	926–32211; RRID: AB_621843
Virus strains
D614	Goethe University	SARS-CoV-2/FFM1, GenBank: MT358638
G614	Goethe University	SARS-CoV-2/FFM7, GenBank: MT358643
Alpha	Goethe University	SARS-CoV-2/FFM-UK7931/2021, Gen Bank: MZ427280
Beta	Goethe University	SARS-CoV-2/FFM-ZAF1/2021, GenBank: MW822592
Delta	Goethe University	SARS-CoV-2/FFM-IND8424/2021, GenBank: MZ315141
Zeta	Goethe University	SARS-CoV-2/FFMBRA1/2021, GenBank: MW822593
Omicron	Goethe University	SARS-CoV-2/FFM-SIM0550/2021, GenBank: OL800702
SARS-CoV	Goethe University	Cinatl et al., 2004
MERS-CoV	BEI	EMC/2012, NR-44260
HCoV-229E	ATCC	CCL-137
Biological samples
Primary bronchial epithelial cells	Dr. Ruth Olmer	van Wetering et al., 2000
Human induced pluripotent stem cell-derived cardiomyocytes	Prof. Stefanie Dimmeler	Breckwoldt et al., 2017
Primary human hepatocytes	Dr. Florian Vondran	Vondran et al., 2008
Chemicals, peptides, and recombinant proteins
DAPI	Thermo Scientific	D1306
Alexa FluorTM 647 Phalloidin	Invitrogen	#A22287
Kinase inhibitor library L-1200	Selleckchem	Catalog No.L1200
Zosuquidar	MedChemExpress	Cat. No.: HY-15255
Remdesivir	MedChemExpress	Cat. No.: HY-104077
NBD-PE	ThermoFisher	N360
Critical commercial assays
Caspase-Glo® 3/7 Assay Systems	Promega	G8093
Caspase-Glo® 8 Assay Systems	Promega	G8201
Caspase-Glo® 9 Assay Systems	Promega	G8211
CellTiter-Glo Luminescent Cell Viability Assay	Promega	G7571
QIAamp Viral RNA Kit	QIAGEN	Cat. No. / ID: 52962
Experimental models: Cell lines
Caco-2A	DSMZ	ACC 169
Caco-2B	Sigma	86010202-1VL
Caco-2C	CLS	300137
Caco-2-F03	RCCL	Available upon request
Caco-2 clones	RCCL	Available upon request
A549-ACE2	Invivogen	a549-hace2
Vero	DSMZ	ACC 33
Calu-3	ATCC	ATCC HTB-55
Oligonucleotides
RdRP_SARSr-F2 (GTG ARA TGG TCA TGT GTG GCG G)	Synthesized by ThermoFisher	NA
RdRP_SARSr-R1 (CAR ATG TTA AAS ACA CTA TTA GCA TA)	Synthesized by ThermoFisher	NA
Recombinant DNA
pEX-A128-RdRP	Dr. Marek Widera	NA
Software and algorithms
Image J	open source software	https://imagej.nih.gov/ij/download.html
CalcuSyn	Biosoft	http://www.biosoft.com/w/calcusyn.htm
GraphPad Prism 9	Graphstats Technologies	https://www.graphstats.net/graphpad-prism